afosa GmbH
Ludwig - Erhard - Ring 3
D-15827 Blankenfelde -
Mahlow, Germany
Tel: +49 (0) 33708-938185
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Anaplasma-ELISA Dog

Enzyme immunoassay for the detection of IgG antibodies to the pathogen of anaplasmosis, Anaplasma phagocytophilum

Anaplasmosis in dogs is a common disease in Europe. Anaplasma-ELISA Dog was designed to replace the standard but time-consuming indirect immunofluorescence antibody test (IFAT) by using the pathogen fixed on slides.

In general antibodies are detectable 10 to 14 days post infection, which is two to five days after the "morulae" can be detected in the dyed blood smear (during the acute stage of the disease). A remarkable rise of antibodies within seven through 21 days is characteristic of an active Anaplasma infection. But a high-level antibody titre persisting for several months does not point out an acute anaplasmosis. If there is suspicion of anaplasmosis then besides the clinical symptoms, the titre dynamic should be monitored.

The sensitivity and the specificity of Anaplasma-ELISA Dog is nearly the same as known from the Anaplasma IFAT. Validation of ELISA using 154 characterized sera from dogs resulted in a sensitivity of 92.5 and specificity up to 97%. Cross reactions to Ehrlichia canis antibodies in dog sera cannot be ruled out.