afosa GmbH
Ludwig - Erhard - Ring 3
D-15827 Blankenfelde -
Mahlow, Germany
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Ehrlichia ELISA-Dog

Enzyme immunoassay for the detection of IgG antibodies to the pathogen of ehrlichiosis in dogs, Ehrlichia canis

The Ehrlichia ELISA Dog has been developed to replace the time-consuming and labor-intensive immunofluorescence antibody assay (IFAT), by using the pathogen fixed on slides.

IgG antibodies against Ehrlichia canis are detected with the Ehrlichia ELISA Dog. In addition to the antibody titre determined, the history, clinic and laboratory diagnostics must be taken into account - also, co-infections with other tick-borne pathogens (multiple tick-borne infections) may overlay the clinical picture and laboratory diagnostic results.

The test validation using 696 defined dog sera for the Ehrlichia-ELISA Dog resulted in a sensitivity of 95.7% and a specificity of 99.2%. According to the literature, in the case of an acute infection, a fourfold IgG titre increase is possible within 7 to 14 days, which is why retesting is recommended for both the IFAT and the ELISA (J. Vet. Int. Med 1996, 10, 271-274 Vet. Microbiol, 2002, 86, 361-368).